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Site‐Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone‐Modified Phosphorothioate DNA
Author(s) -
Wong Ngo Yin,
Zhang Chuan,
Tan Li Huey,
Lu Yi
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201100140
Subject(s) - tetrahedron , dna , bifunctional , dna nanotechnology , dna origami , nanotechnology , materials science , crystallography , nanostructure , biophysics , chemistry , biochemistry , biology , catalysis
Phosphorothioate‐modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and the modification can be programmably placed within the structure.