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A Polymer‐Based Nanopore‐Integrated Microfluidic Device for Generating Stable Bilayer Lipid Membranes
Author(s) -
Kawano Ryuji,
Osaki Toshihisa,
Sasaki Hirotaka,
Takeuchi Shoji
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201090066
Subject(s) - nanopore , microfluidics , membrane , lipid bilayer , bilayer , materials science , polymer , parylene , nanotechnology , chemical engineering , chemistry , engineering , biochemistry , composite material
A parylene nanpore was built into multichannel microfluidic devices and a single protein channel was reconstituted in a bilayer lipid membrane (BLM) on the pore. Although BLMs on micropores used for channel recordings are usually too unstable for the exchange of solutions between the upper and lower sides, the BLMs formed in our nanopores were stable enough to permit solution exchange.