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Fluorophores: Single‐Molecule STED Microscopy with Photostable Organic Fluorophores (Small 13/2010)
Author(s) -
Kasper Robert,
Harke Benjamin,
Forthmann Carsten,
Tinnefeld Philip,
Hell Stefan W.,
Sauer Markus
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201090041
Subject(s) - sted microscopy , fluorophore , microscopy , fluorescence , super resolution microscopy , stimulated emission , resolution (logic) , materials science , fluorescence microscope , optical microscope , photobleaching , chemistry , nanotechnology , optics , scanning electron microscope , laser , physics , composite material , artificial intelligence , computer science
The cover image shows fluorescence images of single immobilized double‐stranded oligonucleotides. With photophysical and ‐chemical properties of fluorophores being the key to breaking the diffraction resolution barrier, the development of far‐field fluorescence nanoscopy depends crucially on improved fluorescent probes. Stimulated emission depletion (STED) microscopy is combined with fluorophore stabilization through a reducing and oxidizing system (ROXS), and a significant improvement of photostability is demonstrated. This improvement can be exploited either for repetitive measurements necessary for 3D or dynamic STED imaging, or for maximizing its resolution. A lateral resolution <30 nm is demonstrated in the raw image data recorded from single organic fluorophores immobilized in an aqueous buffer. For more information, please read the Communication “Single‐Molecule STED Microscopy with Photostable Organic Fluorophores” by P. Tinnefeld, S. W. Hell, M. Sauer, et al., beginning on page 1379 .