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Affibody‐Functionalized Gold–Silica Nanoparticles for Raman Molecular Imaging of the Epidermal Growth Factor Receptor
Author(s) -
Jokerst Jesse V.,
Miao Zheng,
Zavaleta Cristina,
Cheng Zhen,
Gambhir Sanjiv S.
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201002291
Subject(s) - epidermal growth factor receptor , flow cytometry , a431 cells , bioconjugation , epidermal growth factor , colloidal gold , raman spectroscopy , biophysics , surface modification , materials science , nanoparticle , raman scattering , cancer research , receptor , chemistry , nanotechnology , microbiology and biotechnology , cell , biochemistry , biology , molecular medicine , optics , cell cycle , physics
The affibody functionalization of fluorescent surface‐enhanced Raman scattering gold–silica nanoparticles as multimodal contrast agents for molecular imaging specific to epidermal growth factor receptor (EGFR) is reported. This nanoparticle bioconjugate reports EGFR‐positive A431 tumors with a signal nearly 35‐fold higher than EGFR‐negative MDA‐435S tumors. The low‐level EGFR expression in adjacent healthy tissue is 7‐fold lower than in the positive tumors. Validation via competitive inhibition reduces the signal by a factor of six, and independent measurement of EGFR via flow cytometry correlates at R 2 = 0.92.