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Synthesis of Amphiphilic Polythiophene for Cell Imaging and Monitoring the Cellular Distribution of a Cisplatin Anticancer Drug
Author(s) -
Tang Hongwei,
Xing Chengfen,
Liu Libing,
Yang Qiong,
Wang Shu
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201002189
Subject(s) - polythiophene , amphiphile , conjugate , conjugated system , cisplatin , fluorescence , chemistry , drug delivery , combinatorial chemistry , biophysics , materials science , polymer , organic chemistry , conductive polymer , copolymer , biology , mathematical analysis , physics , mathematics , chemotherapy , quantum mechanics , genetics
A new amphiphilic conjugated polythiophene derivative ( PT–Boc ) is synthesized via Suzuki coupling reaction. The amphiphilic characteristic makes it form nanoaggregates in water, in which hydrophobic moieties come together to form the inner core. The polymer shows good photostability and no toxicity to human lung epithelial (A549) cells even at a high concentration (100 μg mL −1 ). It therefore meets the crucial requirement for cellular imaging and other biological applications. The anticancer drug cisplatin is used as a model, and is linked to polythiophene to obtain a conjugate ‘ PT–Pt’ by coordinated interactions between cisplatin and the amine groups of the polythiophene side chain. The PT–Pt can be used for monitoring the cellular distribution of cisplatin by fluorescence microscopy. The amphiphilic polythiophene provides a platform for fluorescent imaging of drugs and biological molecules in living cells.