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Real‐Time Monitoring of Solid‐Phase PCR Using Fiber‐Optic SPR
Author(s) -
Pollet Jeroen,
Janssen Kris P. F.,
Knez Karel,
Lammertyn Jeroen
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201001984
Subject(s) - surface plasmon resonance , polymerase chain reaction , phase (matter) , aptamer , nanotechnology , plasmon , materials science , dna , optical fiber , computer science , computational biology , optoelectronics , physics , gene , nanoparticle , microbiology and biotechnology , biology , telecommunications , genetics , quantum mechanics
Real‐time monitoring of solid‐phase DNA amplification using a surface plasmon resonance sensor (SPR) with gold nanobead signal enhancement is presented. The sensor is applied in the quantification of a DNA aptamer and subsequent melting curve analysis. The system is suited for application in medical and food‐safety diagnostics, and for the study and optimization of solid‐phase polymerase chain reaction.