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Label‐Free Indirect Immunoassay Using an Avidin‐Induced Surface‐Enhanced Raman Scattering Substrate
Author(s) -
Han Xiao Xia,
Chen Lei,
Ji Wei,
Xie Yunfei,
Zhao Bing,
Ozaki Yukihiro
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201001936
Subject(s) - avidin , immunoassay , raman scattering , substrate (aquarium) , biotin , conjugated system , nanotechnology , materials science , nanoparticle , raman spectroscopy , chemistry , analytical chemistry (journal) , chromatography , antibody , optics , biochemistry , physics , medicine , polymer , biology , immunology , composite material , ecology
A label‐free immunoassay based on a simple, surface‐enhanced Raman scattering (SERS) approach is reported. Biotin‐conjugated antibodies are attached to avidin‐aggregated Ag nanoparticles via biotin–avidin biorecognition. More highly sensitive protein detection and more biocompatible protein adsorption are achieved compared to previous relevant methods. Importantly, a SERS intensity ratio of two bands is found to be sensitive to the concentration of corresponding antigens.