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Effective Gene Silencing by Multilayered siRNA‐Coated Gold Nanoparticles
Author(s) -
Lee Seung Koo,
Han Myung Shin,
Asokan Subashini,
Tung ChingHsuan
Publication year - 2011
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201001314
Subject(s) - gene silencing , small interfering rna , colloidal gold , rna interference , protease , nanoparticle , nanotechnology , materials science , biophysics , chemistry , microbiology and biotechnology , rna , gene , biology , biochemistry , enzyme
Small interfering RNA (siRNA) has been widely proposed to treat various diseases by silencing genes, but its delivery remains a challenge. A well controlled assembly approach is applied to prepare a protease‐assisted nanodelivery system. Protease‐degradable poly‐ L ‐lysine (PLL) and siRNA are fabricated onto gold nanoparticles (AuNPs), by alternating the charged polyelectrolytes. In this study, up to 4 layers of PLL and 3 layers of siRNA (sR3P) are coated. Due to the slow degradation of PLL, the incorporated siRNA is released gradually and shows extended gene‐silencing effects. Importantly, the inhibition effect in cells is found to correlate with the number of siRNA layers.