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An On‐Nanoparticle Rolling‐Circle Amplification Platform for Ultrasensitive Protein Detection in Biological Fluids
Author(s) -
Yan Juan,
Song Shiping,
Li Bing,
Zhang Qingzhi,
Huang Qing,
Zhang Hua,
Fan Chunhai
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201001220
Subject(s) - carcinoembryonic antigen , rolling circle replication , nanoparticle , biological fluids , computational biology , nanotechnology , computer science , chemistry , microbiology and biotechnology , chromatography , biology , gene , materials science , biochemistry , cancer , genetics , dna replication
An on‐nanoparticle rolling‐circle amplification (nanoRCA) strategy allows the visual detection of 30 molecules of carcinoembryonic antigen with at least 10 10 ‐fold selectivity over a range of noncognate proteins. The nanoRCA assay is applicable to the detection of two tumor markers in biological fluids: prostate‐specific antigen in spiked sera and interleukin‐8 in saliva.