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A DNA‐Origami Chip Platform for Label‐Free SNP Genotyping Using Toehold‐Mediated Strand Displacement
Author(s) -
Zhang Zhao,
Zeng Dongdong,
Ma Hongwei,
Feng Guoying,
Hu Jun,
He Lin,
Li Can,
Fan Chunhai
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201000908
Subject(s) - genotyping , snp genotyping , dna , snp , single nucleotide polymorphism , chip , nanotechnology , molecular inversion probe , dna microarray , computational biology , biology , genetics , materials science , genotype , computer science , gene , telecommunications , gene expression
A DNA‐origami chip platform for target‐labeling‐free single‐nucleotide polymorphism (SNP) genotyping is presented. DNA capture probes are anchored on an asymmetric map‐shaped DNA‐origami chip and a “toehold”‐mediated strand‐displacement reaction is employed to endow single‐base‐mismatch differentiation specificity. This design leads to a reliable and potentially universal means for SNP genotyping.