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Single‐Molecule STED Microscopy with Photostable Organic Fluorophores
Author(s) -
Kasper Robert,
Harke Benjamin,
Forthmann Carsten,
Tinnefeld Philip,
Hell Stefan W.,
Sauer Markus
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201000203
Subject(s) - sted microscopy , fluorophore , microscopy , materials science , stimulated emission , resolution (logic) , super resolution microscopy , oxidizing agent , optical microscope , molecule , aqueous solution , fluorescence , fluorescence microscope , nanotechnology , chemistry , optics , scanning electron microscope , laser , organic chemistry , physics , composite material , artificial intelligence , computer science
The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single‐molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.