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Kinase‐Actuated Immunoaggregation of Peptide‐Conjugated Gold Nanoparticles
Author(s) -
Gupta Shalini,
Andresen Heiko,
Ghadiali James E.,
Stevens Molly M.
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201000099
Subject(s) - surface plasmon resonance , colloidal gold , conjugated system , nanoparticle , combinatorial chemistry , limiting , chemistry , plasmon , nanotechnology , computer science , materials science , optoelectronics , organic chemistry , polymer , mechanical engineering , engineering
A simple and sensitive one‐step approach for the rapid colorimetric detection of v‐Src kinase using functionalized gold nanoparticles (NPs) in solution is reported. The generic concept operates without labeled ATP‐derivatives, and signals are recorded directly by spectrophotometry. For the first time, surface plasmon resonance spectroscopy is applied to an NP system and compared to a semi‐quantitative NP reaction model that was developed to determine the association rate constants and the rate‐limiting step of the NP agglutination process.