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Polymerase chain reaction: Small 22/2009
Author(s) -
Mi Lijuan,
Wen Yanqin,
Pan Dun,
Wang Yanhong,
Fan Chunhai,
Hu Jun
Publication year - 2009
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200990107
Subject(s) - polymerase chain reaction , polymerase , colloidal gold , dna polymerase , microbiology and biotechnology , dna , chemistry , multiple displacement amplification , polymerase chain reaction optimization , biology , nanotechnology , nanoparticle , nested polymerase chain reaction , biochemistry , materials science , gene , dna extraction
The cover picture illustrates how gold nanoparticles (AuNPs) can dynamically modulate the activity of DNA polymerases and realize hot‐start (HS)‐like effects in polymerase chain reactions (PCR). In particular, when a high‐fidelity Pfu DNA polymerase is combined with AuNPs it becomes a HS version of Pfu with high fidelity and high specificity. That is, AuNPs inactivate the polymerase activity of Pfu at low temperature, resembling an antibody‐based HS PCR, which largely suppresses non‐specific amplification at temperatures between 30 °C and 60 °C, leading to highly specific and sensitive PCR amplification with Pfu. More importantly, the fidelity of Pfu is not sacrificed in the presence of AuNPs. This AuNP‐based HS strategy provides a straightforward and potentially versatile approach to realize high‐performance PCR amplification. For more information, please read the Full Paper “Modulation of DNA Polymerases with Gold Nanoparticles and Their Application in Hot‐Start PCR” by C. Fan, J. Hu, et al., beginning on page 2597 .