Premium
Single‐Molecule FRET Imaging for Enzymatic Reactions at High Ligand Concentrations
Author(s) -
Sugawa Mitsuhiro,
Nishikawa So,
Iwane Atsuko Hikikoshi,
Biju Vasudevanpillai,
Yanagida Toshio
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200901827
Subject(s) - förster resonance energy transfer , ligand (biochemistry) , molecule , chemistry , enzyme , biophysics , nanotechnology , materials science , biochemistry , fluorescence , organic chemistry , receptor , biology , physics , quantum mechanics
Single‐molecule Förster resonance energy transfer (FRET) imaging at micromolar concentrations of an analyte is demonstrated. A quantum dot (QD)–dye donor–acceptor pair permits the approach of physiological levels of fluorescently labeled analytes when imaging molecular dynamics. Single‐molecule FRET imaging of myosin ATP hydrolysis is performed using QDs attached to myosin as a donor enzyme and Cy3‐labeled ATP as an acceptor ligand at micromolar levels (see image).