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Colorimetric Cu 2+ Detection Using DNA‐Modified Gold‐Nanoparticle Aggregates as Probes and Click Chemistry
Author(s) -
Xu Xiaoyang,
Daniel Weston L.,
Wei Wei,
Mirkin Chad A.
Publication year - 2010
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200901691
Subject(s) - click chemistry , oligonucleotide , nanoparticle , copper , duplex (building) , colloidal gold , chemistry , dna , combinatorial chemistry , nanotechnology , materials science , organic chemistry , biochemistry
The colorimetric detection of Cu 2+ is demonstrated using DNA‐modified gold nanoparticle (Au NP) aggregates as probes and a copper‐catalyzed click reaction to initiate the cross‐coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.