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Biofunctionalized, Phosphonate‐Grafted, Ultrasmall Iron Oxide Nanoparticles for Combined Targeted Cancer Therapy and Multimodal Imaging
Author(s) -
Das Manasmita,
Mishra Debasish,
Dhak Prasanta,
Gupta Satyajit,
Maiti Tapas Kumar,
Basak Amit,
Pramanik Panchanan
Publication year - 2009
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200901219
Subject(s) - nanoprobe , materials science , phosphonate , nanotechnology , nanoparticle , folate receptor , drug delivery , iron oxide nanoparticles , targeted drug delivery , molecular imaging , fluorescence lifetime imaging microscopy , conjugate , magnetic resonance imaging , magnetic nanoparticles , combinatorial chemistry , cancer cell , chemistry , fluorescence , cancer , organic chemistry , medicine , mathematics , mathematical analysis , biology , microbiology and biotechnology , quantum mechanics , physics , in vivo , radiology
A novel, inexpensive biofunctionalization approach is adopted to develop a multimodal and theranostic nanoagent, which combines cancer‐targeted magnetic resonance/optical imaging and pH‐sensitive drug release into one system. This multifunctional nanosystem, based on an ultrasmall superparamagnetic iron oxide (USPIO) nanocore, is modified with a hydrophilic, biocompatible, and biodegradable coating of N‐phosphonomethyl iminodiacetic acid (PMIDA). Using appropriate spacers, functional molecules, such as rhodamine B isothiocyanate, folic acid, and methotrexate, are coupled to the amine‐derivatized USPIO–PMIDA support with the aim of endowing simultaneous targeting, imaging, and intracellular drug‐delivering capability. For the first time, phosphonic acid chemistry is successfully exploited to develop a stealth, multifunctional nanoprobe that can selectively target, detect, and kill cancer cells overexpressing the folate receptor, while allowing real‐time monitoring of tumor response to drug treatment through dual‐modal fluorescence and magnetic resonance imaging.