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Restriction‐Enzyme‐Coded Gold‐Nanoparticle Probes for Multiplexed DNA Detection
Author(s) -
Jang KyungJin,
Lee Hyojin,
Jin HuaLan,
Park Yeonhu,
Nam JwaMin
Publication year - 2009
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200901105
Subject(s) - colloidal gold , nanoparticle , dna , multiplexing , nanotechnology , restriction enzyme , materials science , microparticle , biosensor , microbiology and biotechnology , chemistry , biochemistry , biology , computer science , physics , optics , telecommunications
A highly sensitive multiplexed DNA detection assay uses restriction‐enzyme‐encoded, DNA‐modified magnetic microparticle and gold nanoparticle probes combined with dark‐field nanoparticle imaging analysis (see picture). A sensitivity of 100 fM and high specificity of the detection targets are achieved.