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3D Nanometer Tracking of Motile Microtubules on Reflective Surfaces
Author(s) -
Kerssemakers Jacob,
Ionov Leonid,
Queitsch Ute,
Luna Sheila,
Hess Henry,
Diez Stefan
Publication year - 2009
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200801388
Subject(s) - nanometre , tracking (education) , materials science , microtubule , nanotechnology , composite material , biology , psychology , pedagogy , microbiology and biotechnology
Fluorescence interference contrast microscopy in combination with fluorescently labeled, motile microtubules is a versatile tool to scan the geometry of engineered surfaces with nanometer height precision (see image). This method, which is compatible with any kind of reflecting surface, permits dynamic and precise data acquisition in a highly parallel manner using a standard epi‐fluorescence microscope.