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Nanopore Analysis of the Folding of Zinc Fingers
Author(s) -
Stefureac Radu I.,
Lee Jeremy S.
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200800585
Subject(s) - zinc , bumping , nanopore , zinc finger , protein folding , folding (dsp implementation) , crystallography , biophysics , nanotechnology , chemistry , materials science , biochemistry , physics , metallurgy , biology , engineering , gene , transcription factor , electrical engineering , thermodynamics
In the absence of Zn(II) a single Zn‐finger module such as Zif268 is unfolded and can translocate an α ‐hemolysin pore (see image). Upon addition of Zn(II), the protein folds and is too large to translocate so that only bumping events are observed. In contrast, upon addition of Co(II) or Mg(II), there are only small changes to the event profiles.