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Assessing Methods for Blood Cell Cytotoxic Responses to Inorganic Nanoparticles and Nanoparticle Aggregates
Author(s) -
Díaz Belén,
SánchezEspinel Christian,
Arruebo Manuel,
Faro José,
de Miguel Encarnación,
Magadán Susana,
Yagüe Clara,
FernándezPacheco Rodrigo,
Ibarra M. Ricardo,
Santamaría Jesús,
GonzálezFernández África
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200800199
Subject(s) - biocompatibility , cytotoxicity , nanoparticle , reactive oxygen species , internalization , toxicity , cytotoxic t cell , chemistry , nanotechnology , cell , biophysics , materials science , biochemistry , in vitro , biology , organic chemistry
Inorganic nanoparticles (NPs) show great potential for medicinal therapy. However, biocompatibility studies are essential to determine if they are safe. Here, five different NPs are compared for their cytotoxicity, internalization, aggregation in medium, and reactive oxygen species (ROS) production, using tumoral and normal human blood cells. Differences depending on the cell type are analyzed, and no direct correlation between ROS production and cell toxicity is found. Results are discussed with the aim of standardizing the procedures for the evaluation of the toxicity.