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Modified PVA–Fe 3 O 4 Nanoparticles as Protein Carriers into Sperm Cells
Author(s) -
Makhluf Shirly BenDavid,
AbuMukh Riam,
Rubinstein Sara,
Breitbart Haim,
Gedanken Aharon
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200701308
Subject(s) - conjugate , conjugated system , protein kinase c , nanoparticle , iron oxide nanoparticles , covalent bond , western blot , chemistry , antibody , biophysics , materials science , biochemistry , kinase , nanotechnology , biology , organic chemistry , immunology , mathematical analysis , mathematics , gene , polymer
Magnetite nanoparticles conjugated to protein are developed in order to potentially serve as protein carriers into bovine sperm cells. The conjugate comprises iron oxide nanoparticles that are covalently bound to an anti‐protein kinase C (PKC) α antibody. This conjugate can serve for cellular PKC localization and the inhibition of its function. The surface of the nanoparticle is first modified with (3‐aminopropyl) thrimethoxysilane to form a self‐assembled monolayer, and subsequently conjugated with the antibody through amidation between the carboxylic acid end groups on the antibody and the amine groups on the surface of the nanoparticles. The anti‐PKC α localization is proven by fluorescent microscopy and iron staining. The activity of the anti‐PKC α conjugated with the nanoparticle is tested by recognizing PKC α using the Western blot method.