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Stepwise Self‐Assembly of a Protein Nanoarray from a Nanoimprinted Poly(Ethylene Glycol) Hydrogel
Author(s) -
Lee Bong Kuk,
Lee Hea Yeon,
Kim Pilnam,
Suh Kahp Y.,
Seo Jeong Hyun,
Cha Hyung Joon,
Kawai Tomoji
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700865
Subject(s) - ethylene glycol , biotinylation , streptavidin , peg ratio , nanotechnology , materials science , self assembly , nanoimprint lithography , chemistry , biotin , fabrication , organic chemistry , biochemistry , medicine , alternative medicine , finance , pathology , economics
Ultraviolet nanoimprint lithography is used to fabricate nanopatterns of poly(ethylene glycol) (PEG) hydrogel on gold substrates for the construction of protein nanoarrays. A protein nanoarray with a minimum feature size of 100 nm is constructed in the nanowell of the PEG hydrogel by self‐assembly with a biotinylated PEG‐disulfide, streptavidin, and biotinylated antibody, sequentially (see figure), and confirmed by varying the nanowell height between each step in both solution and air.