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Protein Immobilization Without Purification via Dip‐Pen Nanolithography
Author(s) -
Kim Kyung Hee,
Kim Jung Dong,
Kim Young Jun,
Kang Seong Ho,
Jung Seung Yong,
Jung Hyungil
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700519
Subject(s) - nitrilotriacetic acid , dip pen nanolithography , nanolithography , green fluorescent protein , chemistry , histidine , gene , microbiology and biotechnology , nanotechnology , biochemistry , materials science , biology , amino acid , organic chemistry , chelation , medicine , alternative medicine , pathology , fabrication
Selective immobilization of the histidine (His)‐tagged protein from cell lysates is performed on a nitrilotriacetic acid (NTA)/Ni 2+ nanopattern fabricated by dip‐pen nanolithography (see picture). This pattern and His‐coding gene insertions at the end of the target protein gene allow the construction of protein patterns from lysates without prior purification of the protein. LysN = N‐terminal domain of lysyl‐tRNA synthetase; EGFP = enhanced green fluorescent protein.