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Mesoporous Silica Nanoparticles Improve Magnetic Labeling Efficiency in Human Stem Cells
Author(s) -
Liu HonMan,
Wu SiHan,
Lu ChenWen,
Yao Ming,
Hsiao JongKai,
Hung Yann,
Lin YuShen,
Mou ChungYuan,
Yang ChungShi,
Huang DongMing,
Chen YaoChang
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700493
Subject(s) - mesoporous silica , fluorescein isothiocyanate , mesenchymal stem cell , nanoparticle , endocytosis , silicon dioxide , in vivo , iron oxide nanoparticles , biophysics , mesoporous material , superparamagnetism , chemistry , magnetic nanoparticles , in vitro , nanotechnology , stem cell , fluorescein , materials science , cell , biochemistry , fluorescence , microbiology and biotechnology , biology , magnetic field , physics , magnetization , quantum mechanics , metallurgy , catalysis
Tumblerlike magnetic/fluorescein isothiocyanate (FITC)‐labeled mesoporous silica nanoparticles, Mag‐Dye@MSNs, have been developed, which are composed of silica‐coated core–shell superparamagnetic iron oxide (SPIO@SiO 2 ) nanoparticles co‐condensed with FITC‐incorporated mesoporous silica. Mag‐Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5‐T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag‐Dye@MSN‐treated cells than in SPIO@SiO 2 ‐treated cells. This is the first report that MCM‐41‐type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag‐Dye@MSNs than SPIO@SiO 2 .