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Simple Synthesis of Functionalized Superparamagnetic Magnetite/Silica Core/Shell Nanoparticles and their Application as Magnetically Separable High‐Performance Biocatalysts
Author(s) -
Lee Jinwoo,
Lee Youjin,
Youn Jong Kyu,
Na Hyon Bin,
Yu Taekyung,
Kim Hwan,
Lee SangMok,
Koo YoonMo,
Kwak Ja Hun,
Park Hyun Gyu,
Chang Ho Nam,
Hwang Misun,
Park JeGeun,
Kim Jungbae,
Hyeon Taeghwan
Publication year - 2008
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700456
Subject(s) - tetraethyl orthosilicate , magnetite , superparamagnetism , nanoreactor , materials science , dispersity , nanoparticle , chemical engineering , micelle , magnetic nanoparticles , nanotechnology , organic chemistry , polymer chemistry , chemistry , magnetization , aqueous solution , metallurgy , magnetic field , physics , quantum mechanics , engineering
Uniformly sized silica‐coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one‐pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse‐micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size‐selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.