Premium
Using the Ring‐Shaped Protein TRAP to Capture and Confine Gold Nanodots on a Surface.
Author(s) -
Heddle Jonathan G.,
Fujiwara Isamu,
Yamadaki Hirokazu,
Yoshii Shigeo,
Nishio Kazuaki,
Addy Christine,
Yamashita Ichiro,
Tame Jeremy R. H.
Publication year - 2007
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700400
Subject(s) - nanodot , materials science , nanotechnology , transmission electron microscopy
The cavity of the toroidal protein TRAP (trp RNA‐binding attenuation protein) is modified to capture gold nanodots in solution. By engineering a titanium‐binding peptide onto one surface of the ring it is also possible to bind it specifically and tightly to a TiO 2 surface. TRAP bound in this way is then used to capture gold nanodots and attach them to prepared surfaces. Gold‐protein complexes are observed using atomic force microscopy and transmission electron microscopy. The modified TRAP is used to build gold nanodots into the SiO 2 layer of a metal oxide semiconductor. This is the first use of a ring protein, rather than the more commonly used spherical protein cages, to constrain nanodots to a surface. This method is an important addition to the current range of bionanotechnology tools and may be the basis for future, multicomponent electronic devices.