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Monitoring Single‐Cell Infectivity from Virus‐Particle Nanoarrays Fabricated by Parallel Dip‐Pen Nanolithography
Author(s) -
Vega Rafael A.,
Shen Clifton K.F.,
Maspoch Daniel,
Robach Jessica G.,
Lamb Robert A.,
Mirkin Chad A.
Publication year - 2007
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200700244
Subject(s) - nanolithography , dip pen nanolithography , virus , nanotechnology , particle (ecology) , infectivity , green fluorescent protein , materials science , computer science , chemistry , virology , biology , gene , fabrication , biochemistry , medicine , ecology , alternative medicine , pathology
Sting like a bee: Nanoarrays of infectious virus particles, encoded with EGFP, are patterned by dip‐pen nanolithography and exposed to a solution of cells. Upon infection, infected cells produce the EGFP protein, generating a green fluorescence signal that allows one to monitor the cellular infection process in real time (as seen in the optical image). These results suggest that antibody‐immobilized virus particles retain their biological activity. Scale bar: 35μm.