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Characterization of Nanostructured Surfaces Generated by Reconstitution of the Porin MspA from Mycobacterium smegmatis
Author(s) -
Wörner Michael,
Lioubashevski Oleg,
Basel Matthew T.,
Niebler Sandra,
Gogritchiani Eliso,
Egner Nicole,
Heinz Christian,
Hoferer Jürgen,
Cipolloni Michela,
Janik Katharine,
Katz Evgeny,
Braun Andre M.,
Willner Itamar,
Niederweis Michael,
Bossmann Stefan H.
Publication year - 2007
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200600559
Subject(s) - monolayer , materials science , dielectric spectroscopy , mycobacterium smegmatis , colloidal gold , electrochemistry , copper , electrode , self assembled monolayer , chemical engineering , chemistry , nanotechnology , nanoparticle , engineering , metallurgy , mycobacterium tuberculosis , medicine , tuberculosis , pathology
Nanostructures with long‐term stability at the surface of gold electrodes are generated by reconstituting the porin MspA from Mycobacterium smegmatis into a specially designed monolayer of long‐chain lipid surfactant on gold. Tailored surface coverage of gold electrodes with long‐chain surfactants is achieved by electrochemically assisted deposition of organic thiosulfates (Bunte salts). The subsequent reconstitution of the octameric‐pore MspA is guided by its extraordinary self‐assembling properties. Importantly, electrochemical reduction of copper(II) yields copper nanoparticles within the MspA nanopores. Electrochemical impedance spectroscopy, reflection electron microscopy, and atomic force microscopy (AFM) show that: 1) the MspA pores within the self‐assembled monolayer (SAM) are monodisperse and electrochemically active, 2) MspA reconstitutes in SAMs and with a 10‐nm thickness, 3) AFM is a suitable method to detect pores within SAMs, and 4) the electrochemical reduction of Cu 2+ to Cu 0 under overpotential conditions starts within the MspA pores.