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Parallel Manipulation of Bifunctional DNA Molecules on Structured Surfaces Using Kinesin‐Driven Microtubules
Author(s) -
Dinu Cerasela Zoica,
Opitz Jörg,
Pompe Wolfgang,
Howard Jonathon,
Mertig Michael,
Diez Stefan
Publication year - 2006
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200600112
Subject(s) - microtubule , bifunctional , dna origami , kinesin , nanotechnology , dna , biophysics , nanobiotechnology , nanoelectronics , molecule , chemistry , materials science , microbiology and biotechnology , biology , biochemistry , organic chemistry , nanoparticle , catalysis
We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin‐coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof‐of‐principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.