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Glass‐Bead‐Based Parallel Detection of DNA Using Composite Raman Labels
Author(s) -
Jin Rongchao,
Cao Y. Charles,
Thaxton C. Shad,
Mirkin Chad A.
Publication year - 2006
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200500322
Subject(s) - microbead (research) , raman spectroscopy , raman scattering , materials science , oligonucleotide , nanoparticle , colloidal gold , composite number , multiplexing , absorption (acoustics) , nanotechnology , dna , optics , chemistry , computer science , physics , telecommunications , biochemistry , composite material
Gold nanoparticles are co‐functionalized with multiple types of Raman‐dye‐labeled oligonucleotides (see picture) to prepare composite Raman labels for DNA targets that are used in a glass‐microbead‐based multiplexed DNA detection system in conjunction with surface‐enhanced Raman scattering (SERS) spectroscopy. The narrow bands of the Raman spectra of the dye‐labeled nanoparticles allow for greater degrees of multiplexing when compared to the broad absorption and emission bands of molecular fluorophores.