Rapid Detection of  Escherichia coli  by Using Antibody‐Conjugated Silver Nanoshells | Zendy

Kalele Suchita A. | Zendy; Kundu Anita A. | Zendy; Gosavi Suresh W. | Zendy; Deobagkar Dileep N. | Zendy; Deobagkar Deepti D. | Zendy; Kulkarni Sulabha K. | Zendy

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Rapid Detection of Escherichia coli by Using Antibody‐Conjugated Silver Nanoshells

Author(s) -

Kalele Suchita A.,

Kundu Anita A.,

Gosavi Suresh W.,

Deobagkar Dileep N.,

Deobagkar Deepti D.,

Kulkarni Sulabha K.

Publication year - 2006

Publication title -

small

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.785

H-Index - 236

eISSN - 1613-6829

pISSN - 1613-6810

DOI - 10.1002/smll.200500286

Subject(s) - nanoshell , surface plasmon resonance , escherichia coli , materials science , nanotechnology , silver nanoparticle , conjugated system , nanoparticle , chemistry , polymer , biochemistry , composite material , gene

Silver shells of 20 nm thickness have been deposited on silica particles of 200 nm diameter with narrow size distribution. Silver nanoshells dispersed in water exhibit a strong surface plasmon resonance band at 443 nm. This band was found to be very sensitive to rabbit immunoglobulin G antibodies, which were anchored on the nanoshells. These in turn could be utilized to detect the presence of small (≈5) to large numbers (≈10 9 ) of Escherichia coli in water. The protocol presented here proves to be a specific, rapid, reliable, and inexpensive method to detect E. coli.

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