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Rapid Detection of Escherichia coli by Using Antibody‐Conjugated Silver Nanoshells
Author(s) -
Kalele Suchita A.,
Kundu Anita A.,
Gosavi Suresh W.,
Deobagkar Dileep N.,
Deobagkar Deepti D.,
Kulkarni Sulabha K.
Publication year - 2006
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200500286
Subject(s) - nanoshell , surface plasmon resonance , escherichia coli , materials science , nanotechnology , silver nanoparticle , conjugated system , nanoparticle , chemistry , polymer , biochemistry , composite material , gene
Silver shells of 20 nm thickness have been deposited on silica particles of 200 nm diameter with narrow size distribution. Silver nanoshells dispersed in water exhibit a strong surface plasmon resonance band at 443 nm. This band was found to be very sensitive to rabbit immunoglobulin G antibodies, which were anchored on the nanoshells. These in turn could be utilized to detect the presence of small (≈5) to large numbers (≈10 9 ) of Escherichia coli in water. The protocol presented here proves to be a specific, rapid, reliable, and inexpensive method to detect E. coli.