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Selective Loading of Kinesin‐Powered Molecular Shuttles with Protein Cargo and its Application to Biosensing
Author(s) -
Ramachandran Sujatha,
Ernst KarlHeinz,
Bachand George D.,
Vogel Viola,
Hess Henry
Publication year - 2006
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200500265
Subject(s) - kinesin , biotinylation , streptavidin , microtubule , molecular motor , motor protein , protein filament , nanotechnology , chemistry , biophysics , microbiology and biotechnology , materials science , biotin , biochemistry , biology
A new shuttle mission : The design of molecular shuttles, which integrate the motor protein kinesin to propel microtubules (the associated polymeric filament), requires a general strategy to achieve selective cargo binding. We describe the in situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge and demonstrate that selective binding of antigens can be detected via a double‐antibody sandwich assay on the moving shuttle.