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Active Capture and Transport of Virus Particles Using a Biomolecular Motor‐Driven, Nanoscale Antibody Sandwich Assay
Author(s) -
Bachand George D.,
Rivera Susan B.,
CarrollPortillo Amanda,
Hess Henry,
Bachand Marlene
Publication year - 2006
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.200500262
Subject(s) - microtubule , kinesin , covalent bond , virus , nanoscopic scale , nanoparticle , microsphere , nanotechnology , antibody , chemistry , biophysics , materials science , chemical engineering , virology , microbiology and biotechnology , biology , organic chemistry , engineering , immunology
Virus particles are captured and transported using kinesin‐driven, antibody‐functionalized microtubules. The functionalization was achieved through covalent crosslinking, which consequently enhanced the microtubule stability. The capture and transport of the virus particles was subsequently demonstrated in gliding motility assays in which antibody‐coated microtubules functioned as capture elements, and antibody‐coated microspheres served as fluorescent reporters (see Figure).