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A Fluorescent Probe for The Visible Colorimetric Detection of Tyrosinase
Author(s) -
Li Yanan,
Deng Bing,
Yang Shaoxiang,
Tian Hongyu,
Liu Yongguo,
Sun Baoguo
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202102473
Subject(s) - detection limit , colorimetry , fluorescence , chemistry , linear range , tyrosinase , luminescence , luminescent measurements , catechol , substrate (aquarium) , chromatography , quantitative analysis (chemistry) , materials science , biochemistry , optoelectronics , optics , enzyme , biology , ecology , physics
A fluorescent probe (Probe 1) for the visible colorimetric detection of tyrosinase (Tyr) was developed. The quantitative range of detection of Tyr activity detected was 0 to 800 u/mL with the detection limit (LOD) was 5.00 u/mL. The probe solution emitted increasing yellow‐green luminescence with increasing Tyr activity, which was observed under a 365 nm UV lamp. In addition, the green (G) and red (R) values of these photos had a linear relationship with the activity of Tyr. Probe 1 could be used for the quantitative and quantification detection of Tyr activity in real fruits samples using a smartphone. Colorimetry with catechol as the substrate was used to determine the accuracy of this method. This work shows that Probe 1 can be used for the quantitative detection of Tyr using just a smartphone.
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