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A Novel Electrochemical Immunosensor For Sulfadimidine Detection Based On Staphylococcal Protein A−AuNPs/Ag−GO−Nf Modified Electrode[] **
Author(s) -
Wang Aiping,
Zhang Chenyang,
You Xiaojuan,
Zhou Jingming,
Chen Yumei,
Liang Chao,
Ma Dongdong,
Liu Dan,
Zhang Gaiping
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202102260
Subject(s) - sulfadimidine , detection limit , chemistry , electrode , biosensor , electrochemistry , nanocomposite , colloidal gold , linear range , graphene , redox , nanotechnology , nanoparticle , combinatorial chemistry , nuclear chemistry , chromatography , materials science , inorganic chemistry
Sulfadimidine (SM2) is a kind of synthetic sulfadiazine antibiotics. Excessive use of SM2 in animal breeding can lead to animal‐derived food residues, which will bring potential risks to human health. Herein, a sensitive label‐free electrochemical immunosensor using silver nanoparticles (AgNPs) as redox probe was developed for the specific detection of SM2. Firstly, the Ag−GO nanocomposites were prepared by in‐situ reduction of AgNPs on graphene oxide (GO) surface with glucose. Gold nanoparticles (AuNPs) were reduced on the surface of Ag−GO nanocomposites by permanent electrodeposition. Staphylococcal protein A (SPA) was dripped onto the AuNPs modified electrode to absorb antibody (anti‐SM2). Herein, GO provided a large surface area for assembling a large number of AgNPs with redox activity. AuNPs were used to immobilize SPA and amplify the electrochemical signal. In addition, SPA with antibody targeting function was introduced to improve the efficiency of immune response. Under optimal conditions, the label‐free immunosensor showed a linear range of 63 pg/mL to 20 ng/mL with a limit of detection (LOD) of 3 pg/mL (S/N=3). Finally, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other veterinary drug residues in animal derived food.