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Real‐Time Detection of Fibrinogen via Imprinted Recognition Sites
Author(s) -
Çimen Duygu,
Üzek Recep,
Günaydın Serdar,
Denizli Adil
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202101942
Subject(s) - biosensor , surface plasmon resonance , detection limit , fibrinogen , chromatography , molecular imprinting , fourier transform infrared spectroscopy , adsorption , materials science , chemistry , analytical chemistry (journal) , selectivity , nanotechnology , nanoparticle , biochemistry , chemical engineering , organic chemistry , engineering , catalysis
In this study, the fibrinogen imprinted surface plasmon resonance (SPR) biosensor was developed for the monitoring of fibrinogen levels in patient serum samples using a microcontact imprinting method. The fibrinogen imprinted and non‐imprinted polymeric films on the SPR chip surface were prepared by the microcontact imprinting approach and characterized by ellipsometer, contact angle measurements, and Fourier transform infrared spectroscopy. The limit of detection and quantification values of the fibrinogen imprinted SPR biosensors in the linear range of 0.05–9.0 mg/mL were 0.00066 and 0.00219 mg/mL, respectively. In all kinetic analyzes, the response time was 11 min for equilibration, adsorption, and desorption cycles. The selectivity studies of SPR biosensors were performed in the presence of fibrinogen, myoglobin, hemoglobin, and immunoglobulin G in a competitive manner. For the validation studies of the fibrinogen imprinted SPR biosensor, the fibrinogen level of diluted patient serum samples was determined by enzyme‐linked immunosorbent assay (ELISA). The experimental results show that the serum samples of patients in high and low‐risk groups were identified with the recovery of about % 97–99 according to both SPR biosensor and ELISA analysis results.