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“Turn‐on” Fluorescence Chemosensor Based Probing of Cu 2+ with Excellent Sensitivity: Experimental Study, DFT Calculations and Application in Living Cells and Natural Waters
Author(s) -
Savran Tahir,
Nihan Karuk Elmas Sukriye,
Akin Geyik Gonul,
Bostanci Aykut,
Aydin Duygu,
Nur Arslan Fatma,
Sadi Gökhan,
Yilmaz Ibrahim
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202101060
Subject(s) - fluorescence , coumarin , chemistry , density functional theory , chelation , photochemistry , computational chemistry , inorganic chemistry , organic chemistry , physics , quantum mechanics
Developing selective, precise, and rapid determination methods for Cu 2+ sensing is of great significance for biological systems, public health, and environmental protection. Hence, a new coumarin‐based fluorescent chemosensor 3‐((E)‐(((E)‐3,5‐di‐tert‐butyl‐2‐hydroxybenzylidene)hydrazono) methyl)‐7‐(diethylamino)‐2H‐chromen‐2‐one ( HBAC ) for Cu 2+ sensing in MeOH:H 2 O (80 : 20, v:v, pH=7 PBS buffer) media has been constructed. HBAC responded to Cu 2+ with high selectivity and exhibited a relatively low LOD of 7.0 nM and ∼22‐fold fluorescent enhancement at 461 nm with the addition of Cu 2+ (0.0‐5.0 μM). The reaction mechanism of the HBAC ‐Cu 2+ system has been fully examined by MALDI‐TOF‐MS, FT‐IR, and time dependent‐density functional theory (TD‐DFT) calculations. The sensing mechanism was occurred due to the chelation enhanced fluorescence (CHEF) and inhibition of PET phenomena. Furthermore, the chemosensor HBAC for Cu 2+ sensing in living systems and natural spring water samples has been successfully utilized, suggesting that the prepared HBAC is an appropriate chemosensor for examining Cu 2+ in biological and environmental systems.