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The Effect of (3‐Mercaptopropyl)trimethoxysilane (MPS) Coating on the Genetic Detection Performance of Quartz Crystal Microbalance‐Dissipation (QCM‐D) Biosensor: Novel Intact Double‐Layered Surface Modification on QCM‐D
Author(s) -
Soysaldı Furkan,
Soylu Mehmet Çağrı
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202100739
Subject(s) - quartz crystal microbalance , biosensor , detection limit , coating , materials science , sensitivity (control systems) , signal (programming language) , analytical chemistry (journal) , chemistry , adsorption , nanotechnology , chromatography , organic chemistry , electronic engineering , computer science , engineering , programming language
Biosensor's ability depends on both platform performance and surface modification. In mass sensitive biosensors, a stable coating with molecular structural dynamism increases sensitivity. Also, its orientation and smoothness directly affect the performance of cross‐linker molecules and receptors. In this study, the performance of intact double‐layered (ID) coating used on QCM−D for the first time in DNA detection of MPS was investigated. The sensitivity of the QCM−D with ID‐MPS was compared to the uncoated. At the concentrations of 10 10 and 10 13 copies/mL, the method with ID‐MPS was approximately 6.4 times more sensitive. A shift of 1.22(±0.42) Hz was observed at the detection limit at 10 8 copies/mL, while it was 0.19(±0.05) Hz in the negative control. Thus, a signal‐to‐noise‐ratio greater than 3, which is a measure of signal quality in biosensors, was exceeded with a ratio of 3.33, and it was shown that the detection signal at the sensitivity limit was sufficient. In addition, the two methods were compared visually with atomic force microscopy in terms of the amount and distribution of captured molecules, and it was determined that the ID‐MPS was more efficient.