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Radio‐ and Fluorescent‐Labeling of Rituximab Based on the Inverse Electron Demand Diels‐Alder Reaction
Author(s) -
Fernanda García María,
Souza Junqueira Mara,
Silva Mororó Janio,
Camacho Ximena,
Paula Faria Daniele,
Godoi Carneiro Camila,
Gallazzi Fabio,
Chammas Roger,
Quinn Thomas,
Cabral Pablo,
Cerecetto Hugo
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202100042
Subject(s) - bioorthogonal chemistry , bioconjugation , chemistry , in vivo , in vitro , tetrazine , combinatorial chemistry , biochemistry , click chemistry , organic chemistry , biology , microbiology and biotechnology
The bioorthogonal reaction between [1,2,4,5] tetrazines with trans ‐cyclooctene through the inverse electron demand Diels‐Alder (IEDDA) has been described as powerful bioconjugation tool. In this work, we explore the IEDDA as a modular conjugation strategy for in vitro and in vivo labeling of Rituximab for the generation of radioactive and fluorescently label immunoconjugates. The strategy allowed the generation, in vitro and in vivo , of conjugated Rituximab with cyanine 5 and 7 and the gamma emmiter technetium‐99m.