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Immobilization of Active Substances in Food Using Self‐Organized Patterned Porous Film via Breath Figure Approach
Author(s) -
Zhang Simin,
Peng Bo,
Li Meng,
Diao Huayu,
Wang Xingyu,
Zhao Weiping,
Lin Wei,
Sun Na,
Lin Songyi
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202004827
Subject(s) - thermostability , porosity , materials science , nanotechnology , wetting , chemical engineering , porous medium , immobilized enzyme , chromatography , enzyme , chemistry , organic chemistry , composite material , engineering
Abstract Micropattern of bioactive substances in food, such as functional protein and enzyme immobilization, play a crucial role in catalysis, proteomics, tissue engineering, nutrition transportation and biological detection. Currently, various techniques have been reported for the immobilization of bioactive substances on solid but the fabricated methods are often not facile and universal. In this study, we overcome these problems to localized capture ovalbumin (OVA) and trypsin (Try) efficiently by fabricating patterned porous film via simple breath figure approach. Upon optimization of preparation conditions, ordered honeycomb porous film were obtained. We confirmed the morphology and investigated thermostability and surface wettability of porous film. The immobilization of OVA and Try was confirmed by confocal laser scanning microscopy and SDS‐polyacrylamide gel electrophoresis, respectively. To our best knowledge, this work is the first report on patterning and trapping of food protein and enzyme via self‐organized patterned porous film. Our findings are essential for establishing a new convenient and efficient route to design food protein/enzyme‐trapped porous film materials, which opens a new sustainable way for food protein/enzyme immobilization and expected to used in the food industry, biological applications, and so on.