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The Application of Ethanolic Ultrasonication to Ameliorate the Triterpenoid Content Extracted from Vietnamese Ganoderma lucidum with the Examination by Gas Chromatography
Author(s) -
Dat Tran Do,
Viet Nguyen Duc,
My Phan Le Thao,
Linh Nguyen Thi,
Thanh Vuong Hoai,
Linh Nguyen Thi Thuy,
Ngan Nguyen Thi Kim,
Linh Ngo Thi Thuy,
Nam Hoang Minh,
Phong Mai Thanh,
Hieu Nguyen Huu
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202004242
Subject(s) - extraction (chemistry) , dpph , chromatography , chemistry , abts , sonication , solvent , high performance liquid chromatography , fourier transform infrared spectroscopy , gas chromatography , antioxidant , organic chemistry , chemical engineering , engineering
In this study, to investigate the extraction of triterpenoids from the Vietnamese red G. lucidum by ultrasound‐assisted extraction (UAE) method using ethanol as a solvent, the determination of the four single factors influence on the triterpenoid content including liquid‐to‐solid ratio, extraction power, extraction temperature, and extraction time was carried out for the performance of experiments on simultaneous effects by Central Composite design. The results showed that based on ultraviolet‐visible spectroscopy analysis, the highest yield of triterpenoids could be achieved with value of 18.3751 mg/g under optimal extraction conditions including extraction time of 60 min, ultrasonic power of 360 W, liquid‐to‐solid ratio of 38 : 1 mL/g, and ultrasonic temperature of 48 °C. To determine the characteristics of the obtained extract, the Gas chromatography‐mass spectrometry, the Fourier‐transform infrared spectroscopy, scanning electron microscope, and the thermogravimetric analyzer were employed in this study. Besides, for antioxidant capacity evaluation, the DPPH free radical scavenging assay, H 2 O 2 scavenging assay, and the ABTS radical cation decolorization assay were carried out whereas the anticancer evaluation results denoted the potential for anticancer agents of G. lucidum extract. Therefore, it could be concluded that UAE is a promising method for triterpenoid extraction with antioxidant properties.