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On‐Site Testosterone Biosensing for Doping Detection: Electrochemical Immunosensing via Functionalized Magnetic Nanoparticles and Screen‐Printed Electrodes
Author(s) -
Sanli Serdar,
Moulahoum Hichem,
Ghorbanizamani Faezeh,
Gumus Zinar Pinar,
Timur Suna
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202004204
Subject(s) - biosensor , cyclic voltammetry , materials science , electrode , magnetic nanoparticles , detection limit , nanoparticle , nanotechnology , analytical chemistry (journal) , electrochemistry , chromatography , chemistry
Point‐of‐care approaches are in constant need of novel and highly performant portable devices. Doping is a constant issue seen in the athletic field, and a need for mobile and trustable methods for detection are highly sought. The current work proposes a portable biosensor based on a screen‐printed electrode (SPE) and functionalized magnetic particles for testosterone detection. The functionalized magnetic particles were characterized with SEM and the designed biosensor was analyzed amperometrically (Pulse Voltammetry (DPV), Cyclic Voltammetry (CV), and Electrochemical Impedance Spectrometry (EIS)). Results demonstrate linearity between 50–1000 ng/mL with a LOD of 23.68 ng/mL. Q‐TOF/MS method was performed to compare with the biosensor over serum and urine. Furthermore, the selectivity of the biosensor was tested in the presence of various interferents. The designed biosensor shows good potential in doping detection and prevention of illicit use due to the versatility of application that it covers.