Premium
A Flexible DNA Biosensor for Breast Cancer Marker BRCA1 Detection
Author(s) -
Wang Qi,
Zhang Huanyu,
Shen Xiaohui,
Wang Fenghe,
Mao Chun
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202003933
Subject(s) - electrode , breast cancer , biosensor , polyethylene terephthalate , materials science , detection limit , electrical conductor , nanotechnology , substrate (aquarium) , optoelectronics , cancer , chemistry , medicine , chromatography , composite material , biology , ecology
Early detection of breast cancer is of great significance to improve the prognosis and survival rate of breast cancer. However, how to detect breast cancer 1 gene mutation (BRCA1) efficiently in early stage is still a great challenge. In the past few decades, more and more attention has been paid to the design of a biosensor. In this work, A method for rapid detection of specific BRCA1 using silver nanowires modified polyethylene terephthalate (PET) film as a disposable electrode was proposed. With a high aspect ratio, AgNWs distributed in the PET film were easy to form a conductive network as a flexible electrode. A well‐conductive gold sputtering layer can prevent AgNW from falling off to provide a conductive and stable electrode substrate. The Au‐HRP can effectively reflect the hybridization matching phenomenon of target. Results indicated this strategy we proposed had a low detection limit with different concentrations of complementary DNA with limit of detection of 5.3×10 −15 M in the range from 5.0×10 −10 M to 5.0×10 −7 M. Due to the high sensitivity, simplicity and cheap price, arbitrary cut, AgNWs/PET film becomes a promising electrode material for quantification of low concentration biological component in early diagnosis.