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Detection of AFP by Electrochemical Immunosensor Based on Ag/Fe 3 O 4 /g‐C 3 N 4
Author(s) -
Zhang Mengmeng,
Cao Kaihang,
Mei Lisha,
Wang Xiao,
Liao Xiaochen,
Qiao Xiuwen,
Hong Chenglin
Publication year - 2021
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202003896
Subject(s) - detection limit , materials science , graphitic carbon nitride , reproducibility , nuclear chemistry , electrochemistry , analytical chemistry (journal) , graphite , cyclic voltammetry , chemistry , chromatography , photocatalysis , metallurgy , electrode , catalysis , biochemistry
Herein, a novel electrochemical immunosensor was prepared to detect cancer alpha‐fetoprotein (AFP). An iron oxide/graphite‐phase carbon nitride/silver (Fe 3 O 4 −g‐C 3 N 4 −Ag) composite was used as the carrier. Metal‐free graphitic carbon nitrides (g‐C 3 N 4 ) have been recently recognized as high‐performance photocatalysts; however, their large‐scale applications are limited because of their poor electronic properties and low dispersion. Fe 3 O 4 particles were incorporated in the composite to improve the conductivity and dispersibility of g‐C 3 N 4 . Silver nanoparticles were prepared through UV irradiation and did not involve the use of reducing agents. A signal probe was prepared from Fe 3 O 4 −g‐C 3 N 4 −Ag and acetylene black. Cyclic voltammetry was conducted to detect AFP. Under optimized conditions, the proposed immunosensor exhibited excellent analytical abilities. The dynamic response range of the AFP concentration was 0.0001–80 ng ⋅ mL −1 . The detection limit was relatively low at 0.078 pg ⋅ mL −1 (S/N=3). The immunosensor also exhibited high specificity and good reproducibility. The results showed that the proposed immunosensor can be successfully used to detect AFP in human serum samples.