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Fluorescence In Situ Hybridization of 16S rRNA in Escherichia coli Using Multiple Photo‐Cross‐Linkable Probes
Author(s) -
Fujimoto Kenzo,
Watanabe Nanami
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202003343
Subject(s) - rna , fluorescence , in situ , protein secondary structure , cooperativity , fluorescence in situ hybridization , sequence (biology) , ribosomal rna , in situ hybridization , biophysics , biology , computational biology , microbiology and biotechnology , chemistry , genetics , gene , optics , biochemistry , messenger rna , physics , organic chemistry , chromosome
RNA forms various secondary structures depending on its sequence in cells; the influence of secondary structures must be considered when detecting, regulating, or manipulating RNA. In this study, we utilized the cooperativity of multiple photo‐cross‐linkable assistant probes to evaluate photo‐cross‐linking for RNAs with complex secondary structures. We succeeded in increasing the fluorescence intensity 39.9 times in the region where detection was difficult owing to the formation of a complex secondary structure in the conventional fluorescence in situ hybridization method. This method enables the detection, regulation, and manipulation of RNA that forms various structures depending on the sequence.