Premium
Facile Preparation of a Nanocomposite with Bifunctional Groups for the Separation and Analysis of Phosphopeptides in Human Saliva
Author(s) -
Liu Bin,
Lu Yujie,
Yan Yinghua,
Wang Chenlu,
Ding ChuanFan,
Tang Keqi
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202002091
Subject(s) - nanocomposite , bifunctional , selectivity , substrate (aquarium) , saliva , chemistry , carbon nanotube , composite number , layer (electronics) , chemical engineering , chromatography , materials science , nanotechnology , organic chemistry , composite material , catalysis , biochemistry , oceanography , geology , engineering
A composite material was well prepared (magCNT@PDA@NH 2 ‐MIL‐125@mTiO 2 ) with magnetic carbon nanotubes as a substrate and polydopamine as good hydrophilicity mid‐layer by facile procedure. Compared with single functional composite (magCNT@PDA@MIL‐125‐NH 2 and magCNT@PDA@mTiO 2 ), magCNT@PDA@NH 2 ‐MIL‐125@mTiO 2 exhibits higher sensitivity (4 fmol), better selectivity (nonphosphopeptides:phosphopeptodes=1 : 1000), as well as more excellent size‐exclusion effect towards phosphopeptides under the same conditions. Meanwhile, 33 phosphopeptides were selectively detected derived from human saliva, this result shows that the nanocomposite has the potential to enrich phosphopeptides from complex biological samples.