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Electrochemical Characterization of Redox Probes at Gold Screen‐Printed Electrodes: Efforts towards Signal Stability
Author(s) -
Ribeiro José A.,
Silva Elisa,
Moreira Patrícia S.,
Pereira Carlos M.
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.202001411
Subject(s) - electrode , electrochemistry , biosensor , redox , materials science , cyclic voltammetry , biomolecule , amperometry , analytical chemistry (journal) , surface plasmon resonance , nanotechnology , chemistry , chromatography , nanoparticle , metallurgy
In this work, three universally used redox probes in amperometric biosensing devices, [Fe(CN) 6 ] 3− /[Fe(CN) 6 ] 4− , Ru[(NH 3 ) 6 ] 3+ , and ferrocenedimethanol (FDM), were selected to evaluate the stability of electrochemical signals provide by the reporting systems. Studies were carried out at disposable gold screen‐printed electrode (AuSPE) biosensing platforms, commonly used for screening chemical and biological relevant biomolecules. Firstly, electrochemical combined‐surface plasmon resonance (eSPR) studies were performed to evaluated adsorption reversibility and/or formation of redox probe complexes at the bare gold surface when routinely used electrochemical techniques, namely cyclic voltammetry (CV) and square‐wave voltammetry (SWV), are recorded. Then, the results obtained were compared with those obtained at the AuSPE under the same electrochemical conditions. Based on our findings, best experimental conditions, including the type of electrochemical technique used, are speculated for each reporting system in order to improve the analytical signal stability. Finally, a methodology based on SWV technique was applied to modified electrodes to provide a simple and easy tool to ensure diffusion controlled permeability of probes thorough the films to electrode surface.