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Enzyme‐Assisted Cyclic Signal Amplification by Using Carbon Nanomaterials for Hg 2+ Detection
Author(s) -
Liu Wei,
Yang Lijuan,
Yu Kaili,
Li Yongyang
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201904946
Subject(s) - fluorescence , graphene , carbon nanotube , chemistry , detection limit , tap water , metal ions in aqueous solution , exonuclease , nanomaterials , hydrolysis , linear range , metal , carbon fibers , nuclear chemistry , analytical chemistry (journal) , enzyme , materials science , nanotechnology , chromatography , organic chemistry , composite number , dna polymerase , physics , quantum mechanics , environmental engineering , engineering , composite material
Abstract A novel and simple fluorescence method for Hg 2+ recognition and detection has been developed based on T‐Hg 2+ ‐T complex and exonuclease III (Exo III) assisted cyclic signal amplification. Graphene oxide (GO) and carbon nanotubes (CNTs) were used to quench the fluorescence FAM‐labelled single strand DNA (FAM‐ssDNA). The optimum conditions, including GO and CNTs concentrations, enzyme concentration and enzymatic hydrolysis time, were studied. The linear range, regression equation and metal ion selectivity were further studied and compared. The recovery rates of Hg 2+ in tap water and urine were between 93.83–103.5%.