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Two‐Photon Detection of Organotin Schiff Base Complexes in Cancer Cells
Author(s) -
BerronesReyes Jessica C.,
MuñozFlores Blanca M.,
UscangaPalomeque Ashanti Concepción,
Santillán Rosa,
Del AngelMosqueda Casiano,
Nobis David,
Cochrane Max A.,
Magennis Steven W.,
JiménezPérez Víctor M.
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201904816
Subject(s) - hela , fluorescence , schiff base , two photon excitation microscopy , chemistry , cancer cell , cancer , microbiology and biotechnology , cell , biochemistry , stereochemistry , biology , physics , genetics , quantum mechanics
The early detection of cancer cells and their visualization before and after surgery are essential for successful pre‐ and post‐operative disease management. Although fluorescence imaging is a sensitive and versatile tool that is finding increasing use in clinical applications, there is a lack of tumour‐targeting fluorophores. We report here a family of fluorescent Schiff base organotin dyes ( 1 : Et 2 N−L‐SnPh 2 , 2 : Et 2 N−L‐SnBu 2 , 3 : MeO−L‐SnPh 2 , 4 : MeO−L‐SnBu 2 , 5 : HO−L‐SnPh 2 , and 6 : HO−L‐SnBu 2 , where L=2‐hydroxybenzylidene‐4‐hydroxybenzhydrazine). In addition to one‐photon‐excited fluorescence, efficient two‐photon excitation was demonstrated in compounds 1 – 4 . Two of the compounds ( 5 and 6 ), both with hydroxyl substituents, were capable of selective accumulation in HeLa cells, allowing differentiation from normal cells (periodontal ligament cells). Compounds 1 and 3 showed excellent cancer cell staining (HeLa) using two‐photon bioimaging, which is promising for biomedicine applications.