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““Turn‐On” Sensing Behaviour of an In Situ Generated Fluorescein‐Based Probe and Its Preferential Selectivity of Sodium Hypochlorite over tert ‐Butyl Hydroperoxide in Lung Adenocarcinoma Cells”
Author(s) -
Sandhu Navjot,
Saproo Sheetanshu,
Naidu Srivatsava,
Singh Atul P.,
Kumar Kamlesh,
Singh Ashish P.,
Yadav Rajesh K.
Publication year - 2020
Publication title -
chemistryselect
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.437
H-Index - 34
ISSN - 2365-6549
DOI - 10.1002/slct.201903843
Subject(s) - sodium hypochlorite , in situ , fluorescence , oxidizing agent , chemistry , fluorescein , reactive oxygen species , hypochlorite , molecule , selectivity , fluorescence microscope , oxygen , photochemistry , turn (biochemistry) , sodium fluorescein , biophysics , biochemistry , inorganic chemistry , organic chemistry , catalysis , biology , physics , quantum mechanics
Reactive Oxygen species (ROS) are well known for their oxidizing behaviour but it differs in fluorescein derivatives chemistry. With t ‐BuOOH, the probe F shows its unique turn‐off sensing (A), while with NaOCl probe shows turn‐on sensing property (B). In this study, it was observed that in situ generated species formed by the reaction of probe (F) with t ‐BuOOH is a unique molecule, having high turn‐on behaviour in presence of sodium hypochlorite (≈500 times). Further, fluorescence microscopy imaging using adenocarcinoma cells, A549 cells revealed that in‐situ generated molecule, could be used as an efficient fluorescent probe for detecting NaOCl in living cells.